Anti-Dopamine beta Hydroxylase antibody (ab96615) at 1/5000 dilution + HepG2 whole cell lysate at 30 µg
Immunofluorescence analysis of Dopamine beta Hydroxylase in paraformaldehyde fixed HeLa cell, using ab96615 at a 1/200 dilution. Lower image: merged with DNA probe.
mmunohistochemical analysis of Dopamine beta Hydroxylase in paraffin embedded SW480 xenograft, using ab96615 at a 1/500 dilution.
ab96615 staining Dopamine beta Hydroxylase in human colorectal tumor tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0.3% Triton X-100 in PBS and blocked with 5% goat serum for 1 hour at 24°C. Samples were incubated with primary antibody (1/1000) for 3 hours at 24°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG monoclonal (1/2000) was used as the secondary antibody.See Abreview