Anti-Dopamine Receptor D1 antibody
| Name | Anti-Dopamine Receptor D1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab40653 |
| Prices | $379.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF IHC-P |
| Species Reactivities | Rat, Human, Mouse, Bovine, Pig, Monkey, Monkey |
| Antigen | Synthetic peptide: MDGTGLVVERDFS linked to a carrier protein by a carboxyl terminal Cysteine residue linker |
| Blocking Peptide | Dopamine Receptor D1 peptide |
| Description | Rabbit Polyclonal |
| Gene | DRD1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab40653 (1:500) staining Dopamine Receptor D1 in human cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of cytoplasmic and membrane regions in the fibrous glial cells.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
All lanes : Anti-Dopamine Receptor D1 antibody (ab40653) at 1/1000 dilutionLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Rat Cortex Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
Developmental changes in human dopamine neurotransmission: cortical receptors and - Developmental changes in human dopamine neurotransmission: cortical receptors and
Rothmond DA, Weickert CS, Webster MJ. BMC Neurosci. 2012 Feb 15;13:18.
Adolescent social defeat increases adult amphetamine conditioned place preference - Adolescent social defeat increases adult amphetamine conditioned place preference
Burke AR, Watt MJ, Forster GL. Neuroscience. 2011 Dec 1;197:269-79.