Western blot analysis of extracts of various cell lines using Atg9A (D4O9D) Rabbit mAb.
Western blot analysis of extracts from RD cells, transfected with 100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-) or SignalSilence ® Atg9A siRNA I #7051 (+), using Atg9A (D4O9D) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The Atg9A (D4O9D) Rabbit mAb confirms silencing of Atg9A expression while the β-Actin (D6A8) Rabbit mAb is used as a loading control.
Immunoprecipitation of Atg9A from Hep G2 cell extracts using Rabbit (DA1E) mAb IgG XP ® Isotype control #3900 (lane 2) or Atg9A (D4O9D) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Atg9A (D4O9D) Rabbit mAb.