All lanes : Anti-DPP3 antibody - Catalytic domain (ab56108) at 1/1000 dilutionLane 1 : Whole cell lysate from human fetal lung HFL1 cells (treated with SF) at 35 µlLane 2 : Whole cell lysate from human fetal lung HFL1 cells (treated with SF) at 30 µlLane 3 : Whole cell lysate from human fetal lung HFL1 cells (treated with SF) at 25 µlLane 4 : Whole cell lysate from human hepatoma SK-HEP-1 cells (treated with IL1-beta) at 15 µlLane 5 : Whole cell lysate from human hepatoma SK-HEP-1 cells (treated with IL1-beta) at 10 µl