Anti-DRAM antibody
| Name | Anti-DRAM antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab68987 |
| Prices | $398.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF FC WB IHC-P IHC-F |
| Species Reactivities | Mouse, Human |
| Antigen | A synthetic peptide from human DRAM conjugated to an immunogenic carrier protein |
| Description | Rabbit Polyclonal |
| Gene | DRAM1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-DRAM antibody (ab68987) at 1/300 dilutionLane 1 : PC12 cell lysateLane 2 : PC12 cell lysate with Immunising peptide
Flow Cytometry analysis of DRAM using ab68987.Human monocytes were fixed in paraformaldehyde and permeabilized. ab68987 was used at a 1/1000 dilution. The secondary used was an Alexa-Fluor 488 conjugated chicken anti-rabbit IgG (H+L) polyclonal, used at a 1/2000 dilution.
ab68987 staining DRAM in human monocytes by Immunocytochemistry/ Immunofluorescence.Cells were fixed, permeabilize, blocked with 2% BSA for 1 hour at 25°C and then incubated with ab68987 at a 1/250 dilution for 4 hours at 25°C. The secondary used was an Alexa-Fluor 488 conjugated chicken anti-rabbit IgG (H+L) used at a 1/1000 dilution.See Abreview
ab68987 staining DRAM in Mouse RAW 264.7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 in 2% BSA and blocked with 2% Milk for 1 hour at 22°C. Samples were incubated with primary antibody (1/500 in PBS + 2% BSA) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Chicken anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. WGA = Wheat Germ Agglutinin See Abreview
Immunohistochemical analysis of Human placenta tissue, taken from patients with intrauterine growth restriction (IUGR). DRAM (green) was stained with ab68987 (1/100 dilution) overnight at 4°C. An AlexaFluor®488-conjugated goat anti-mouse IgG (1/200) was used as the secondary antibody. Nuclei were stained with DAPI (blue).
Product References
Phosphorylated AKT inhibits the apoptosis induced by DRAM-mediated mitophagy in - Phosphorylated AKT inhibits the apoptosis induced by DRAM-mediated mitophagy in
Liu K, Shi Y, Guo XH, Ouyang YB, Wang SS, Liu DJ, Wang AN, Li N, Chen DX. Cell Death Dis. 2014 Feb 20;5:e1078.
Autophagy in the human placenta throughout gestation. - Autophagy in the human placenta throughout gestation.
Hung TH, Hsieh TT, Chen SF, Li MJ, Yeh YL. PLoS One. 2013 Dec 13;8(12):e83475.
Increased autophagy in placentas of intrauterine growth-restricted pregnancies. - Increased autophagy in placentas of intrauterine growth-restricted pregnancies.
Hung TH, Chen SF, Lo LM, Li MJ, Yeh YL, Hsieh TT. PLoS One. 2012;7(7):e40957.