Example of CXCL11 standard curve in Sample Diluent 25BS. Background-subtracted data values (mean +/- SD) are graphed.
Observed CXCL11 levels in individual donor normal Human serum (n=10). Mean Human CXCL11 is 131 pg/mL and values fall within expected normal reference ranges (WHO).
THP-1 cells were cultured in RPMI supplemented with 10% fetal calf serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 µg/mL streptomycin. Cells were stimulated for 8 hours with or without 1 µg/mL of human IFN-gamma recombinant protein. An aliquot of supernatant was removed and assayed for native CXCL11 protein levels. The concentrations of CXCL11 were interpolated from the calibration curve and corrected for sample dilution. The mean CXCL11 level was 58 pg/mL in untreated cells and 9,624 pg/mL in IFN-gamma treated cells.
Human peripheral blood cells (1x106 cells/mL) were cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 mg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 10 mg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed for endogenous CXCL11 levels.
Observed CXCL11 levels in pooled donor normal Human plasma. Mean Human CXCL11 values fall within expected normal reference ranges (WHO).
Titration of cell culture supernatant from THP-1 cells stimulated with IFN-gamma within the working range of the assay. Background-subtracted data values (mean +/- SD, n = 3) are graphed.
Example of CXCL11 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.