Cyt C Reductase (Complex III) Human Profiling ELISA Kit
| Name | Cyt C Reductase (Complex III) Human Profiling ELISA Kit |
|---|---|
| Supplier | Abcam |
| Catalog | ab124537 |
| Category | ELISA Kit |
| Prices | $448.00 |
| Sizes | 1 x 96 tests |
| Assay Type | Sandwich (qualitative) |
| Sample Type | Tissue Extracts, Cell Lysate |
| Detection | Colorimetric |
| Sensitivity | 4 µg/ml |
| Range | 6 µg/ml - 200 µg/ml |
| Format | Microplate |
| Applications | ELISA |
| Species Reactivities | Human |
| Gene | UQCRC1 |
| Supplier Page | Shop |
Product images
Example control sample curve of serially titrated HepG2 extract in the working range of the assay.
Human HepG2 cells were cultured in chloramphenicol for 6 days to ensure a significant effect on mitochondrial DNA replication and mitochondrial protein translation, respectively. The antibiotic inhibited mitochondrial protein translation and assembly of Complexes I and IV but had no significant effect on Complex II, III or V.
Human HepG2 cells were cultured in NARTI Zalcitabine (ddC) for 6 days to ensure a significant effect on mitochondrial DNA replication and mitochondrial protein translation, respectively. The drug reduced mitochondrial DNA levels and hence mitochondrial protein expression. As a consequence the assembly of Complexes I, III and IV were severely affected. Note that loss of the two small mitochondrial DNA encoded subunits of Complex V (ATP synthase) does not affect overall assembly. Interestingly an increase in Complex II was induced as a consequence of I, III, IV loss possibly to up regulate mitochondrial citric acid cycle function.