A431 cells were treated with recombinant human EGF at 37°C for 20 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.
A431 cells were treated or untreated with 100 ng/ml recombinant human EGF for 10 min. Cell lysates were analyzed using this phosphoELISA.
A431 cells were treated or untreated with 100 ng/ml recombinant human EGF for 10 min. Cell lysates were analyzed by Western Blot.
The A431 cells were treated with recombinant human EGF for 10 minutes to induce phosphorylation of EGFR. Serial dilutions of lysates were analyzed in this ELISA.
The A431 cells were treated with recombinant human EGF for 10 minutes to induce phosphorylation of EGFR. Serial dilutions of lysates were analyzed by Western blot. Immunoblots were incubated with anti-phospho-EGFR (Ser 1070).