Background-subtracted data values (mean +/- SD) are graphed.
Titration of MCF7 lysate within the working range of the assay. Background subtracted data from triplicate measurements are plotted.
Interpolated values of ERK1 (MAPK3) are plotted for the indicated cell lines based on a lysate load of 250 µg/mL.
ERK1 (MAPK3) protein (included with this kit) and ERK2 (MAPK1) protein (ab43625) were loaded within the dynamic range of the assay (45 ng/mL) in triplicates. The assay was found to react exclusively against ERK1 (MAPK3).
Comparison of ERK1 (MAPK3) expression between the ELISA and Western blot using different cell lines. Background subtracted data from duplicate measurements are plotted. Signal saturates for C6 (Rat cell line) and NIH3T3 (mouse cell line) in the ELISA assay when lysate is loaded at 500 µg/mL. Western blot samples were loaded at 30 µg/well.