PhosphoTracer ERK 1/2 (pT202/Y204) + total ERK ELISA Kit (ab119659) used in Sandwich ELISA.
Using the ERK 1/2 assay kits, a significant reduction of ERK 1/2 phosphorylation is detected in A431 cells treated with AG1478 for 3 hours compared with untreated cells, while only a small change in total ERK levels is observed.
Using Western blot, a significant reduction of ERK 1/2 phosphorylation is detected in A431 cells treated with AG1478 for 3 hours compared with untreated cells, while only a small change in total ERK levels is observed.