Representative Standard Curve using ab136950.
Concentrations of mouse Grp94 were measured in lysates from either control or heat-shocked 3T3 cells. Cell lysates were prepared using Extraction Reagent, and were serially diluted into assay buffer prior to the assay.
Dose-response curves from cell lysates diluted into assay buffer (using the MRD) were compared to the recombinant Canine Grp94 standard curve. Parallelism indicates antibody-binding characteristics of the native and standard proteins are similar, allowing accurate determination of analyte.