GSK3 beta (GSK3B) ELISA Kit

• Supplier: Abcam
• Catalog: ab123454
• Category: ELISA Kit
• Assay Type: Sandwich (quantitative)
• Sample Type: Cell Lysate
• Detection: Colorimetric
• Sensitivity: 0.7 ng/ml
• Range: 1.56 ng/ml - 100 ng/ml
• Format: Microplate
• Applications: ELISA
• Species Reactivities: Mouse, Rat, Human
• Gene: GSK3B

Product images

Demonstration of assay specificity. Western blot using the GSK3B detector antibody (ab32391). HeLa cells treated with DMSO show a doublet band due to endogenous phosphorylation of the protein in this cell line. Wortmannin, a PI3-K inhibitor, dephosphorylates AKT and in-turn dephosphorylates GSK3B. Dephosphorylation removes the doublet band observed with vehicle treatment only.

Example standard curve. A serially diluted recombinant GSK3B protein (ab63193) in the working range of the assay