GSK3 beta (pS9) + AKT 1/2/3 (pS473) + p70S6K (pT389) + S6RP (pS240/244) PhosphoTracer ELISA Kit

• Supplier: Abcam
• Catalog: ab119676
• Category: ELISA Kit
• Prices: $573.00
• Sizes: 1 x 96 tests
• Assay Type: Semi-quantitative
• Sample Type: Cell culture extracts
• Detection: Fluorescent
• Format: Microplate
• Applications: ELISA
• Species Reactivities: Mouse, Human, Rat
• Gene: RAPGEF4

Product images

Using the GSK3beta assay kits or Western blot, GSK3beta phosphorylation at Ser9 is detected in insulin-treated MCF7 cells (+), compared with cells treated with UCN-01 (-).

Using the AKT 1/2/3 assay kits, a significant increase in AKT 1/2/3 phosphorylation at Ser473 is detected in MCF7 cells treated with insulin for 15 minutes (+), compared with untreated cells (-).

Using the p70S6K assay kits, a significant reduction of p70S6K phosphorylation at Thr389 is detected in MCF7 cells treated with wortmannin (-) for 3 hours, compared with untreated cells (+).

Using the S6RP assay kits or Western blot, S6RP phosphorylation at Ser240/244 is detected in insulin-treated MCF7 cells (+), compared with MCF7 cells treated with a combination of rapamycin, UCN-01 and U0126 (-).