Background-subtracted data values (mean +/- SD) are graphed.
Background-subtracted data values (mean +/- SD, n=2) are graphed.
Background-subtracted data values (mean +/- SD, n=2) are graphed.
Cell extracts (250 µg/mL) prepared from un-induced (control) and heat-shocked (HS, 30 min at 42ºC, followed by recovery period, as indicated, at 37ºC) cells were analyzed with this kit. The concentrations of HSP70 were interpolated from data values using HSP70 standard curve and graphed in units (U) of HSP70 per mg of extract (mean, n=2).
Cell extracts (40 µg) prepared from un-induced (lane 3, Jurkat; lane 7, HeLa) cells or 30 min at 42ºC heat-shocked, then recovered at 37ºC for variable time periods (lane 4, 1 hour; lane 5, 4 hours; lane 6, 6.5 hours) Jurkat cells were analyzed by Western blotting using the capture antibody of this kit. The Human recombinant HSP70 protein standard was analyzed as well (lane 2). Note that this antibody detects a single band of 70 kDa and that the HSP70 levels obtained by this analysis correlate with the result obtained with the use of this kit.
Cell extracts (40 µg) prepared from un-induced (lane 3, Jurkat; lane 7, HeLa) cells or 30 min at 42ºC heat-shocked, then recovered at 37ºC for variable time periods (lane 4, 1 hour; lane 5, 4 hours; lane 6, 6.5 hours) Jurkat cells were analyzed by Western blotting using the detector antibody of this kit. The Human recombinant HSP70 protein standard was analyzed as well (lane 2). Note that this antibody detects a single band of 70 kDa and that the HSP70 levels obtained by this analysis correlate with the result obtained with the use of this kit.