![Chromatin Immunoprecipitation: QuickChip (with control) ChIP Kit [NBP2-29902] - Human MCF-7 cells were fixed for 10 minutes with 1% formaldehyde. The cells were lysed and the chromatin sonicated (200-1000 bp fragments). Chromatin was immunoprecipitated using DNMT3b mAb, (Lane 2) and a no antibody control (Lane 3). The PCR amplification was performed, with primers specific to the progesterone receptor (PR) promoter region, as follows: 94C 5 min, 94C 30 s, 59C 30 s 40 cycles, 72C 30 s, 72C 10 min. Following PCR, 20 ul of each sample was analyzed on a 2% agarose gel and visualized by UV-illumination following ethidium bromide staining. PCR product was observed in the DNMT3b mAb ChIP (Lane 2) and not in the control (Lane 3).](http://www.bioprodhub.com/system/product_images/ak_products/5/sub_1/405_QuickChip-(with-control)-ChIP-Kit-Chromatin-Immunoprecipitation-NBP2-29902-img0003.jpg)
Chromatin Immunoprecipitation: QuickChip (with control) ChIP Kit [NBP2-29902] - Human MCF-7 cells were fixed for 10 minutes with 1% formaldehyde. The cells were lysed and the chromatin sonicated (200-1000 bp fragments). Chromatin was immunoprecipitated using DNMT3b mAb, (Lane 2) and a no antibody control (Lane 3). The PCR amplification was performed, with primers specific to the progesterone receptor (PR) promoter region, as follows: 94C 5 min, 94C 30 s, 59C 30 s 40 cycles, 72C 30 s, 72C 10 min. Following PCR, 20 ul of each sample was analyzed on a 2% agarose gel and visualized by UV-illumination following ethidium bromide staining. PCR product was observed in the DNMT3b mAb ChIP (Lane 2) and not in the control (Lane 3).
![QuickChip (with control) ChIP Kit [NBP2-29902]](http://www.bioprodhub.com/system/product_images/ak_products/5/sub_1/406_QuickChip-(with-control)-ChIP-Kit-NBP2-29902-img0001.jpg)
QuickChip (with control) ChIP Kit [NBP2-29902]