Western Blot: Mitochondrial Extraction Kit [NBP2-29448] - Mouse liver cells were fractionated using . 20 ug of cytosolic (lane 1) and mitochondrial (lane 2) extracts were probed with anti-Cytochrome C antibody, and anti-Actin antibody (loading control). extraction kit efficiently separated the two fractions and no Cytochrome C (specific to mitochondria) was detected in the cytosolic extract