![Western Blot: beta Galactosidase IHC Kit [NBP2-29546] - 5-bromo-4-chloro-3-indolyl-Beta-D-galactopyranoside (X-Gal) Chromogeneic substrate. Beta-Gal cleavage of the glycosidic linkage in X-Gal (1) produces a colorless indoxyl monmer, 5-bromo-4-chloro-indolyl (2). Nonenzymatic oxidation and dimerization occur in the presence of ferric and ferrous ions resulting in 5,5'-di-bromo-44'-dichloro-indigo, a stable and insoluble dark blue halogenated indigocompound (3).](http://www.bioprodhub.com/system/product_images/ak_products/5/sub_1/566_beta-Galactosidase-IHC-Kit-Western-Blot-NBP2-29546-img0008.jpg)
Western Blot: beta Galactosidase IHC Kit [NBP2-29546] - 5-bromo-4-chloro-3-indolyl-Beta-D-galactopyranoside (X-Gal) Chromogeneic substrate. Beta-Gal cleavage of the glycosidic linkage in X-Gal (1) produces a colorless indoxyl monmer, 5-bromo-4-chloro-indolyl (2). Nonenzymatic oxidation and dimerization occur in the presence of ferric and ferrous ions resulting in 5,5'-di-bromo-44'-dichloro-indigo, a stable and insoluble dark blue halogenated indigocompound (3).
![Immunohistochemistry: beta Galactosidase IHC Kit [NBP2-29546] - B-Gal activity of retroviral mediated expression of the LacZ gene. 293-10A1 cells were co-transfected with 5 ug each of pCLMFG-LacZ and pCL-Eco plasmids. Two days post transfection, 10 ul of supernatant was used to infect NIH-3T3 cells grown on chamber slides. B-Gal activity was demonstrated according to the protocol described](http://www.bioprodhub.com/system/product_images/ak_products/5/sub_1/567_beta-Galactosidase-IHC-Kit-Immunohistochemistry-NBP2-29546-img0007.jpg)
Immunohistochemistry: beta Galactosidase IHC Kit [NBP2-29546] - B-Gal activity of retroviral mediated expression of the LacZ gene. 293-10A1 cells were co-transfected with 5 ug each of pCLMFG-LacZ and pCL-Eco plasmids. Two days post transfection, 10 ul of supernatant was used to infect NIH-3T3 cells grown on chamber slides. B-Gal activity was demonstrated according to the protocol described