IFN gamma measured in cell culture supernatants from control or treated (48 hours; 2% PHA-M; LifeTechnologies) PBMCs (duplicates +/- SD).
IFN gamma measured in cell culture supernatants from control or stimulated (48 hours with 50 ng x mL-1 PMA (ab120297) and 1 μM ionomycin (ab120116)) Jurkat cells. Control sample was below limit of detection (<0.57 pg x mL-1) (duplicates +/- SD).
IFN gamma measured in human biological fluids; mouse and rat samples (plasma and serum) were below limit of detection (duplicates +/- SD).
Dilution curves of IFN gamma (ab46048) and NIBSC standard (87/586). One ng of standard IFN gamma corresponds to 12.3 (+/- 1.3) IU NIBSC 87/586. Background signal subtracted (duplicates; +/- SD).
Representative Standard Curve using ab46048