Native human IgG3 protein was measured in serum diluted 1:150,000 in sample diluent NS prior to a 2-fold dilution series in Sample Diluent NS. Native Human IgG3 protein was measured in citrate plasma (1:125,000), EDTA plasma (1:150,000), and heparin plasma (1:200,000) in a 2-fold dilution series in Sample Diluent NS. The interpolated dilution factor corrected values are graphed (mean +/- SD).
Ten individual healthy male donors and one pooled sample (n = 50) were evaluated for the presence of IgG3 in serum using this assay. Results were interpolated from the standard curve in Sample Diluent NS and corrected by sample dilution (1:200,000). In the 10 individual donors, the mean level of IgG3 is 381.44 µg/mL with a range of 173.71 – 746.69 µg/mL and a standard deviation of 159.71 µg/mL.
Background-subtracted data values (mean +/- SD) are graphed.