Dilution curves of mouse IL-1 beta (ab100705) and NIBSC standard (93/668). One ng of standard mouse IL-1 beta corresponds to 34.6 (+/-2.1) AU NIBSC 93/668. Background signal subtracted (duplicates; +/- SD).
IL-1 beta measured in mouse tissue lysates (mg of extracted protein; duplicates +/- SD).
IL-1 beta measured in lysates from control cells, or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297), with the addition of 1 ug x mL-1 of LPS (Sigma) (P/L) for the last 6 hours (duplicates +/- SD).
Representative standard curve using ab100705