Rabbit anti-MAD1 Antibody, Affinity Purified

Name	Rabbit anti-MAD1 Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0092
Prices	$323.50
Sizes	100 µl
Applications	ICC/IF
Species Reactivities	Human
Gene	MAD1L1
Supplier Page	Shop

Product images

<B>Immunohistochemistry</B><BR/>Rabbit Anti-MAD1 Antibody, Affinity Purified: <B>Cat. No. PLA0092</B>: Detection of Human MAD1 by Immunohistochemistry. Sample: FFPE section of Human prostate carcinoma. Antibody: Affinity purified Rabbit Anti-MAD1 (<B>Cat. No. PLA0092</B>) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB

Immunohistochemistry
Rabbit Anti-MAD1 Antibody, Affinity Purified: Cat. No. PLA0092: Detection of Human MAD1 by Immunohistochemistry. Sample: FFPE section of Human prostate carcinoma. Antibody: Affinity purified Rabbit Anti-MAD1 (Cat. No. PLA0092) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-MAD1 Antibody, Affinity Purified: <B>Cat. No. PLA0092</B>: Detection of Human MAD1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (50 μg/lane in A; 50 μg input in B, 1 mg for IP in B) from HeLa cells. In A, HeLa cells were treated with Nocodazole (+) or mock treated (-). Antibody: Affinity purified Rabbit Anti-MAD1 Antibody (<B>Cat. No. PLA0092</B>) used at 0.1 μg/mL for Western Blot (A) and at 1 μg/mg lysate for IP (B). MAD1 was also immunoprecipitated using Rabbit Anti-MAD1 Antibodies recognizing different epitopes using 1 μg/mg lysate. In B, MAD1 in input and immunoprecipitate was detected using Antibody at 0.2 μg/mL. Detection: Chemiluminescence with exposure times of 10 minutes (A) and 5 minutes (B).

Immunoblotting
Rabbit Anti-MAD1 Antibody, Affinity Purified: Cat. No. PLA0092: Detection of Human MAD1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (50 μg/lane in A; 50 μg input in B, 1 mg for IP in B) from HeLa cells. In A, HeLa cells were treated with Nocodazole (+) or mock treated (-). Antibody: Affinity purified Rabbit Anti-MAD1 Antibody (Cat. No. PLA0092) used at 0.1 μg/mL for Western Blot (A) and at 1 μg/mg lysate for IP (B). MAD1 was also immunoprecipitated using Rabbit Anti-MAD1 Antibodies recognizing different epitopes using 1 μg/mg lysate. In B, MAD1 in input and immunoprecipitate was detected using Antibody at 0.2 μg/mL. Detection: Chemiluminescence with exposure times of 10 minutes (A) and 5 minutes (B).