Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).
ImmunoblottingRabbit Anti-MCPH1 Antibody, Affinity Purified:
Cat. No. PLA0099: Detection of Human MCPH1 by Western Blot. Samples: Whole cell lysate (30 μg) from U2OS cells treated with a MCPH1 siRNA or a LacZ siRNA (A) and whole cell lysate (5, 15 and 50 μg) from 293T cells (B). Antibody: Affinity purified Rabbit Anti-MCPH1 Antibody (
Cat. No. PLA0099) used at 1 μg/mL in A and at 1 and 0.4 μg/mL in B. Detection: Chemiluminescence with an exposure time of 30 seconds (A and B).