Rabbit anti-MLL1 Antibody, Affinity Purified

Name	Rabbit anti-MLL1 Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0100
Prices	$323.50
Sizes	100 µl
Applications	ChIP
Species Reactivities	Mouse, Ape, Horse, Marmoset, Human, Monkey, Rat, Chimpanzee, Primate
Gene	KMT2A
Supplier Page	Shop

Product images

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-MLL1 Antibody, Affinity Purified: <B>Cat. No. PLA0100</B>: Detection of Human C-terminal 180 kDa Fragment of MLL1 by Western Blot. Samples: Whole cell lysate from K562 (15 and 50 μg for WB), 293T (Lane T; 50 μg) and HeLa (Lane H; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-MLL1 antibody <B>Cat. No. PLA0100</B> used for WB at 0.4 μg/mL. Detection: Chemiluminescence with exposure time of 3 minutes.

Immunoblotting
Rabbit Anti-MLL1 Antibody, Affinity Purified: Cat. No. PLA0100: Detection of Human C-terminal 180 kDa Fragment of MLL1 by Western Blot. Samples: Whole cell lysate from K562 (15 and 50 μg for WB), 293T (Lane T; 50 μg) and HeLa (Lane H; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-MLL1 antibody Cat. No. PLA0100 used for WB at 0.4 μg/mL. Detection: Chemiluminescence with exposure time of 3 minutes.

<B>Immunoprecipitation</B><BR/>Rabbit Anti-MLL1 Antibody, Affinity Purified: <B>Cat. No. PLA0100</B>: Detection of Human C-terminal 180 kDa Fragment of MLL1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from K562 cells. Antibodies: Affinity purified Rabbit Anti-MLL1 antibody <B>Cat. No. PLA0100</B> for IP used for IP at 6 μg/mg lysate. The C-terminal 180 kDa Fragment of MLL1 was also immunoprecipitated by MLL1 Antibody B. For blotting immunoprecipitated MLL1, <B>PLA0100</B> was used at 1 μg/mL. Detection: Chemiluminescence with exposure time of 10 seconds.

Immunoprecipitation
Rabbit Anti-MLL1 Antibody, Affinity Purified: Cat. No. PLA0100: Detection of Human C-terminal 180 kDa Fragment of MLL1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from K562 cells. Antibodies: Affinity purified Rabbit Anti-MLL1 antibody Cat. No. PLA0100 for IP used for IP at 6 μg/mg lysate. The C-terminal 180 kDa Fragment of MLL1 was also immunoprecipitated by MLL1 Antibody B. For blotting immunoprecipitated MLL1, PLA0100 was used at 1 μg/mL. Detection: Chemiluminescence with exposure time of 10 seconds.