Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).
ImmunoblottingGoat Anti-FUS Antibody, Affinity Purified:
Cat. No. PLA0296: Detection of Human FUS by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 μg) and HeLa (Lane H; 50 μg) cells. Antibodies: Affinity purified goat Anti-FUS Antibody
PLA0296 used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
ImmunoblottingGoat Anti-FUS Antibody, Affinity Purified:
Cat. No. PLA0296: Detection of Mouse FUS by Western Blot. Samples: Whole cell lysate (15 and 50 μg) from NIH3T3 cells. Antibodies: Affinity purified goat Anti-FUS Antibody
PLA0296 used for WB at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
ImmunoprecipitationGoat Anti-FUS Antibody, Affinity Purified:
Cat. No. PLA0296: Detection of Human FUS by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified goat Anti-FUS antibody
Cat. No. PLA0296 used for IP at 6 μg/mg lysate. FUS was also immunoprecipitated by Rabbit Anti-FUS Antibody B. For blotting immunoprecipitated FUS,
PLA0296 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.