Rabbit anti-PCNA Antibody, Affinity Purified

Name	Rabbit anti-PCNA Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0080
Prices	$323.50
Sizes	100 µl
Applications	ICC/IF
Species Reactivities	Human, Guinea Pig, Ape, Mouse, Bovine, Pig, Rabbit, Rat, Dog, Monkey, Sheep, Primate
Gene	PCNA
Supplier Page	Shop

Product images

<B>Immunohistochemistry</B><BR/>Rabbit Anti-PCNA Antibody, Affinity Purified: <B>Cat. No. PLA0080</B>: Detection of Human and Mouse PCNA by Immunohistochemistry. Sample: FFPE section of Human stomach carcinoma (left) and Mouse teratoma (right). Antibody: Affinity purified Rabbit Anti-PCNA (<B>Cat. No. PLA0080</B>) used at a dilution of 1:10,000 (0.1 μg/mL). Detection: DAB

Immunohistochemistry
Rabbit Anti-PCNA Antibody, Affinity Purified: Cat. No. PLA0080: Detection of Human and Mouse PCNA by Immunohistochemistry. Sample: FFPE section of Human stomach carcinoma (left) and Mouse teratoma (right). Antibody: Affinity purified Rabbit Anti-PCNA (Cat. No. PLA0080) used at a dilution of 1:10,000 (0.1 μg/mL). Detection: DAB

<B>Immunofluorescence</B><BR/>Rabbit Anti-PCNA Antibody, Affinity Purified: <B>Cat. No. PLA0080</B>: Detection of Human PCNA by Immunofluorescence. Sample: FFPE section of Human stomach carcinoma. Antibody: Affinity purified Rabbit Anti-PCNA (<B>Cat. No. PLA0080</B>) used at a dilution of 1:4,000 (0.25 μg/mL). Detection: Red-fluorescent Goat Anti-Rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight<SUP>®</SUP> 594 Conjugated used at a dilution of 1:100.

Immunofluorescence
Rabbit Anti-PCNA Antibody, Affinity Purified: Cat. No. PLA0080: Detection of Human PCNA by Immunofluorescence. Sample: FFPE section of Human stomach carcinoma. Antibody: Affinity purified Rabbit Anti-PCNA (Cat. No. PLA0080) used at a dilution of 1:4,000 (0.25 μg/mL). Detection: Red-fluorescent Goat Anti-Rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight^® 594 Conjugated used at a dilution of 1:100.

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-PCNA Antibody, Affinity Purified: <B>Cat. No. PLA0080</B>: Detection of Human PCNA by Western Blot and Immunoprecipitaiton. Samples: A. Whole cell lysate (WCL) from A-431 cells. B. WCL (500 μg for IP; 30 μg for WCL lane) from MDA-MB-468 cells. Antibodies: A. Affinity purified Rabbit Anti-PCNA Antibody (<B>Cat. No. PLA0080</B>) used at 0.1 μg/mL for WB. B. PCNA was immunoprecipitated using the Antibody (<B>Cat. No. PLA0079</B>) or a Mouse monoclonal Antibody to PCNA (mAb). Control mock IP was performed using normal Mouse IgG (M-IgG) and normal Rabbit IgG (R-IgG). Immunoprecipitated PCNA was blotted using the alternate Antibody (each at 1 μg/mL). Detection: Chemiluminescence with an exposure times as indicated (A) or 5 seconds (B).

Immunoblotting
Rabbit Anti-PCNA Antibody, Affinity Purified: Cat. No. PLA0080: Detection of Human PCNA by Western Blot and Immunoprecipitaiton. Samples: A. Whole cell lysate (WCL) from A-431 cells. B. WCL (500 μg for IP; 30 μg for WCL lane) from MDA-MB-468 cells. Antibodies: A. Affinity purified Rabbit Anti-PCNA Antibody (Cat. No. PLA0080) used at 0.1 μg/mL for WB. B. PCNA was immunoprecipitated using the Antibody (Cat. No. PLA0079) or a Mouse monoclonal Antibody to PCNA (mAb). Control mock IP was performed using normal Mouse IgG (M-IgG) and normal Rabbit IgG (R-IgG). Immunoprecipitated PCNA was blotted using the alternate Antibody (each at 1 μg/mL). Detection: Chemiluminescence with an exposure times as indicated (A) or 5 seconds (B).