Rabbit anti-Chk1 Antibody, Affinity Purified

Name	Rabbit anti-Chk1 Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0085
Prices	$357.50
Sizes	100 µl
Applications	ICC/IF
Species Reactivities	Primate, Human, Pig, Dog, Chimpanzee, Ape, Monkey
Gene	CHEK1
Supplier Page	Shop

Product images

<B>Immunohistochemistry</B><BR/>Rabbit Anti-Chk1 Antibody, Affinity Purified: <B>Cat. No. PLA0085</B>: Detection of Human Chk1 by Immunohistochemistry. Sample: FFPE section of Human testicular seminoma. Antibody: Affinity purified Rabbit Anti-Chk1 (<B>Cat. No. PLA0085</B>) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB staining.

Immunohistochemistry
Rabbit Anti-Chk1 Antibody, Affinity Purified: Cat. No. PLA0085: Detection of Human Chk1 by Immunohistochemistry. Sample: FFPE section of Human testicular seminoma. Antibody: Affinity purified Rabbit Anti-Chk1 (Cat. No. PLA0085) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB staining.

<B>Immunocytochemistry</B><BR/>Rabbit Anti-Chk1 Antibody, Affinity Purified: <B>Cat. No. PLA0085</B>: Detection of Human Chk1 by Immunocytochemistry. Samples: NBF-fixed HeLa cells treated with neocarzinostatin. Antibody: Affinity purified Rabbit Anti-Chk1 (<B>Cat. No. PLA0085</B>) used at a dilution of 1:1,000 (1 μg/mL). Detection: Anti-Rabbit IgG-FITC conjugated used at a dilution of 1:100.

Immunocytochemistry
Rabbit Anti-Chk1 Antibody, Affinity Purified: Cat. No. PLA0085: Detection of Human Chk1 by Immunocytochemistry. Samples: NBF-fixed HeLa cells treated with neocarzinostatin. Antibody: Affinity purified Rabbit Anti-Chk1 (Cat. No. PLA0085) used at a dilution of 1:1,000 (1 μg/mL). Detection: Anti-Rabbit IgG-FITC conjugated used at a dilution of 1:100.

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-Chk1 Antibody, Affinity Purified: <B>Cat. No. PLA0085</B>: Detection of Human Chk1 by Western Blot. Samples: Whole cell lysate (20 μg) from HeLa cells. Antibody: Affinity purified Rabbit Anti-Chk1 Antibody (<B>Cat. No. PLA0085</B>) used at the indicated concentrations. Detection: Chemiluminescence with exposure times of 15 seconds and 1 hour.

Immunoblotting
Rabbit Anti-Chk1 Antibody, Affinity Purified: Cat. No. PLA0085: Detection of Human Chk1 by Western Blot. Samples: Whole cell lysate (20 μg) from HeLa cells. Antibody: Affinity purified Rabbit Anti-Chk1 Antibody (Cat. No. PLA0085) used at the indicated concentrations. Detection: Chemiluminescence with exposure times of 15 seconds and 1 hour.