Rabbit anti-ATRIP Antibody, Affinity Purified

Name	Rabbit anti-ATRIP Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0130
Prices	$323.50
Sizes	100 µl
Applications	ICC/IF
Species Reactivities	Human
Gene	ATRIP
Supplier Page	Shop

Product images

<B>Immunohistochemistry</B><BR/>Rabbit Anti-ATRIP Antibody, Affinity Purified: <B>Cat. No. PLA0130</B>: Detection of Human ATRIP by Immunohistochemistry. Sample: FFPE section of Human prostate carcinoma. Antibody: Affinity purified Rabbit Anti-ATRIP (<B>Cat. No. PLA0130</B>) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB staining.

Immunohistochemistry
Rabbit Anti-ATRIP Antibody, Affinity Purified: Cat. No. PLA0130: Detection of Human ATRIP by Immunohistochemistry. Sample: FFPE section of Human prostate carcinoma. Antibody: Affinity purified Rabbit Anti-ATRIP (Cat. No. PLA0130) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB staining.

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-ATRIP Antibody, Affinity Purified: <B>Cat. No. PLA0130</B>: Detection of Human ATRIP by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg for WB; 1 mg for IP, 20% of IP loaded), 293T (Lane T; 50 μg) and Mouse NIH3T3 (Lane M; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-ATRIP Antibody (<B>Cat. No. PLA0130</B>) used for WB at 0.1 μg/mL (A) and 1 μg/mL (B), and used for IP at 3 μg/mg lysate. ATRIP was also immunoprecipitated using Rabbit Anti-ATRIP Antibodies, each of which recognizes a different epitope on Human ATRIP. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).

Immunoblotting
Rabbit Anti-ATRIP Antibody, Affinity Purified: Cat. No. PLA0130: Detection of Human ATRIP by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg for WB; 1 mg for IP, 20% of IP loaded), 293T (Lane T; 50 μg) and Mouse NIH3T3 (Lane M; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-ATRIP Antibody (Cat. No. PLA0130) used for WB at 0.1 μg/mL (A) and 1 μg/mL (B), and used for IP at 3 μg/mg lysate. ATRIP was also immunoprecipitated using Rabbit Anti-ATRIP Antibodies, each of which recognizes a different epitope on Human ATRIP. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).