Rabbit anti-BRD4 Antibody, Affinity Purified

Name	Rabbit anti-BRD4 Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0227
Prices	$649.00
Sizes	100 µl
Applications	ICC/IF
Species Reactivities	Mouse, Human
Gene	BRD4
Supplier Page	Shop

Product images

<B>Immunohistochemistry</B><BR/>Rabbit Anti-BRD4 Antibody, Affinity Purified: <B>Cat. No. PLA0227</B>: Detection of Mouse BRD4 by Immunohistochemistry. Samples: FFPE sections of Mouse renal cell carcinoma. Antibody: Affinity purified Rabbit Anti-BRD4 (<B>Cat. No. PLA0227</B>) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB staining.

Immunohistochemistry
Rabbit Anti-BRD4 Antibody, Affinity Purified: Cat. No. PLA0227: Detection of Mouse BRD4 by Immunohistochemistry. Samples: FFPE sections of Mouse renal cell carcinoma. Antibody: Affinity purified Rabbit Anti-BRD4 (Cat. No. PLA0227) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB staining.

<B>Immunohistochemistry</B><BR/>Rabbit Anti-BRD4 Antibody, Affinity Purified: <B>Cat. No. PLA0227</B>: Detection of Human BRD4 by Immunohistochemistry. Samples: FFPE sections of Human ovarian carcinoma. Antibody: Affinity purified Rabbit Anti-BRD4 (<B>Cat. No. PLA0227</B>) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB staining.

Immunohistochemistry
Rabbit Anti-BRD4 Antibody, Affinity Purified: Cat. No. PLA0227: Detection of Human BRD4 by Immunohistochemistry. Samples: FFPE sections of Human ovarian carcinoma. Antibody: Affinity purified Rabbit Anti-BRD4 (Cat. No. PLA0227) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB staining.

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-BRD4 Antibody, Affinity Purified: <B>Cat. No. PLA0227</B>: Detection of Human BRD4 by Western Blot. Samples: Whole cell lysate (50 μg) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified Rabbit Anti-BRD4 Antibody (<B>Cat. No. PLA0227</B>) used for WB at 0.04 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoblotting
Rabbit Anti-BRD4 Antibody, Affinity Purified: Cat. No. PLA0227: Detection of Human BRD4 by Western Blot. Samples: Whole cell lysate (50 μg) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified Rabbit Anti-BRD4 Antibody (Cat. No. PLA0227) used for WB at 0.04 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

<B>Immunoprecipitation</B><BR/>Rabbit Anti-BRD4 Antibody, Affinity Purified: <B>Cat. No. PLA0227</B>: Detection of Human BRD4 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-BRD4 antibody <B>Cat. No. PLA0227</B> used for IP at 6 μg/mg lysate. BRD4 was also immunoprecipitated by Rabbit Anti-BRD4 Antibody B. For blotting immunoprecipitated BRD4, <B>PLA0227</B> was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.

Immunoprecipitation
Rabbit Anti-BRD4 Antibody, Affinity Purified: Cat. No. PLA0227: Detection of Human BRD4 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-BRD4 antibody Cat. No. PLA0227 used for IP at 6 μg/mg lysate. BRD4 was also immunoprecipitated by Rabbit Anti-BRD4 Antibody B. For blotting immunoprecipitated BRD4, PLA0227 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.