Goat anti-DBC1/p30 DBC Antibody, Affinity Purified

Name	Goat anti-DBC1/p30 DBC Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0305
Prices	$323.50
Sizes	100 µl
Applications	IP
Species Reactivities	Human, Mouse
Gene	CCAR2
Supplier Page	Shop

Product images

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Goat Anti-DBC1/p30 DBC Antibody, Affinity Purified: <B>Cat. No. PLA0305</B>: Detection of Human DBC1/p30 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 μg), HeLa (50 μg), and Jurkat (50 μg) cells. Antibodies: Affinity purified goat Anti-DBC1/p30 Antibody <B>PLA0305</B> used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoblotting
Goat Anti-DBC1/p30 DBC Antibody, Affinity Purified: Cat. No. PLA0305: Detection of Human DBC1/p30 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 μg), HeLa (50 μg), and Jurkat (50 μg) cells. Antibodies: Affinity purified goat Anti-DBC1/p30 Antibody PLA0305 used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

<B>Immunoblotting</B><BR/>Goat Anti-DBC1/p30 DBC Antibody, Affinity Purified: <B>Cat. No. PLA0305</B>: Detection of Mouse DBC1/p30 by Western Blot. Samples: Whole cell lysate (50 μg) from NIH3T3 , Renca, and TCMK-1 cells. Antibodies: Affinity purified goat Anti-DBC1/p30 Antibody <B>PLA0305</B> used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoblotting
Goat Anti-DBC1/p30 DBC Antibody, Affinity Purified: Cat. No. PLA0305: Detection of Mouse DBC1/p30 by Western Blot. Samples: Whole cell lysate (50 μg) from NIH3T3 , Renca, and TCMK-1 cells. Antibodies: Affinity purified goat Anti-DBC1/p30 Antibody PLA0305 used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

<B>Immunoprecipitation</B><BR/>Goat Anti-DBC1/p30 DBC Antibody, Affinity Purified: <B>Cat. No. PLA0305</B>: Detection of Human DBC1/p30 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified goat Anti-DBC1/p30 antibody <B>Cat. No. PLA0305</B> used for IP at 6 μg/mg lysate. DBC1/p30 was also immunoprecipitated by Rabbit Anti-DBC1/p30 Antibody B. For blotting immunoprecipitated Rabbit Anti-DBC1/p30 Antibody B was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.

Immunoprecipitation
Goat Anti-DBC1/p30 DBC Antibody, Affinity Purified: Cat. No. PLA0305: Detection of Human DBC1/p30 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified goat Anti-DBC1/p30 antibody Cat. No. PLA0305 used for IP at 6 μg/mg lysate. DBC1/p30 was also immunoprecipitated by Rabbit Anti-DBC1/p30 Antibody B. For blotting immunoprecipitated Rabbit Anti-DBC1/p30 Antibody B was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.