Rabbit anti-RIF1 Antibody, Affinity Purified

Name	Rabbit anti-RIF1 Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0117
Prices	$323.50
Sizes	100 µl
Applications	ICC/IF
Species Reactivities	Monkey, Human, Chimpanzee, Ape, Primate
Gene	RIF1
Supplier Page	Shop

Product images

<B>Immunohistochemistry</B><BR/>Rabbit Anti-RIF1 Antibody, Affinity Purified: <B>Cat. No. PLA0117</B>: Detection of Human RIF1 by Immunohistochemistry. Sample: FFPE section of Human laryngeal squamous cell carcinoma. Antibody: Affinity purified Rabbit Anti- RIF1 (<B>Cat. No. PLA0117</B> Lot1) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB

Immunohistochemistry
Rabbit Anti-RIF1 Antibody, Affinity Purified: Cat. No. PLA0117: Detection of Human RIF1 by Immunohistochemistry. Sample: FFPE section of Human laryngeal squamous cell carcinoma. Antibody: Affinity purified Rabbit Anti- RIF1 (Cat. No. PLA0117 Lot1) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-RIF1 Antibody, Affinity Purified: <B>Cat. No. PLA0117</B>: Detection of Human RIF1 by Western Blot after Immunoprecipitation. Sample: Whole cell lysate (1 mg/IP reaction; 1/4 of reaction loaded) from 293T cells. Antibodies: Affinity purified Rabbit Anti-RIF1 Antibody (<B>Cat. No. PLA0117</B>) used at 5 μg/mg lysate for IP. RIF1 was also immunoprecipitated using Rabbit Anti-RIF1 Antibodies recognizing different epitopes at 5 μg/mg lysate. Immunoprecipitated RIF1 was blotted using Antibody at 0.4 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoblotting
Rabbit Anti-RIF1 Antibody, Affinity Purified: Cat. No. PLA0117: Detection of Human RIF1 by Western Blot after Immunoprecipitation. Sample: Whole cell lysate (1 mg/IP reaction; 1/4 of reaction loaded) from 293T cells. Antibodies: Affinity purified Rabbit Anti-RIF1 Antibody (Cat. No. PLA0117) used at 5 μg/mg lysate for IP. RIF1 was also immunoprecipitated using Rabbit Anti-RIF1 Antibodies recognizing different epitopes at 5 μg/mg lysate. Immunoprecipitated RIF1 was blotted using Antibody at 0.4 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.