Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).
ImmunoblottingRabbit Anti-DC8 Antibody, Affinity Purified:
Cat. No. PLA0143: Detection of Human DC8 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg for WB; 1 mg for IP, 20% of IP loaded) and 293T (Lane T; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-DC8 Antibody (
Cat. No. PLA0143) used for WB at 0.1 μg/mL (A) and 1 μg/mL (B) and used for IP at 3 μg/mg lysate (B). DC8 was also immunoprecipitated by Rabbit Anti-DC8 Antibodies that recognize different epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).