Rabbit anti-NONO Antibody, Affinity Purified

Name	Rabbit anti-NONO Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0119
Prices	$323.50
Sizes	100 µl
Applications	ICC/IF
Species Reactivities	Dog, Rabbit, Guinea Pig, Human, Rat, Pig, Mouse, Horse, Monkey, Bovine, Primate
Gene	NONO
Supplier Page	Shop

Product images

<B>Immunohistochemistry</B><BR/>Rabbit Anti-NONO Antibody, Affinity Purified: <B>Cat. No. PLA0119</B>: Detection of Human and Mouse NONO by Immunohistochemistry. Sample: FFPE section of Human breast carcinoma (left) and Mouse squamous cell carcinoma (right). Antibody: Affinity purified Rabbit Anti- NONO (<B>Cat. No. PLA0119</B>) used at a dilution of 1:1,000 (0.2 μg/mL) and 1:200 (1 μg/mL). Detection: DAB

Immunohistochemistry
Rabbit Anti-NONO Antibody, Affinity Purified: Cat. No. PLA0119: Detection of Human and Mouse NONO by Immunohistochemistry. Sample: FFPE section of Human breast carcinoma (left) and Mouse squamous cell carcinoma (right). Antibody: Affinity purified Rabbit Anti- NONO (Cat. No. PLA0119) used at a dilution of 1:1,000 (0.2 μg/mL) and 1:200 (1 μg/mL). Detection: DAB

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-NONO Antibody, Affinity Purified: <B>Cat. No. PLA0119</B>: Detection of Human NONO by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg for WB; 1 mg for IP, 1/4 of IP reaction loaded) and 293T (T; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-NONO Antibody (<B>Cat. No. PLA0119</B>) used for WB at 0.04 μg/mL (A) and 1 μg/mL (B) and used for IP at 3 μg/mg lysate. NONO was also immunoprecipitated using an Anti-NONO Antibody that recognizes a different epitope at 3 μg/mg lysate. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).

Immunoblotting
Rabbit Anti-NONO Antibody, Affinity Purified: Cat. No. PLA0119: Detection of Human NONO by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg for WB; 1 mg for IP, 1/4 of IP reaction loaded) and 293T (T; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-NONO Antibody (Cat. No. PLA0119) used for WB at 0.04 μg/mL (A) and 1 μg/mL (B) and used for IP at 3 μg/mg lysate. NONO was also immunoprecipitated using an Anti-NONO Antibody that recognizes a different epitope at 3 μg/mg lysate. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).

<B>Immunoblotting</B><BR/>Rabbit Anti-NONO Antibody, Affinity Purified: <B>Cat. No. PLA0119</B>: Detection of Mouse and Rat NONO by Western Blot. Samples: Whole cell lysate (50 μg) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified Rabbit Anti-NONO Antibody <B>PLA0119</B> used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoblotting
Rabbit Anti-NONO Antibody, Affinity Purified: Cat. No. PLA0119: Detection of Mouse and Rat NONO by Western Blot. Samples: Whole cell lysate (50 μg) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified Rabbit Anti-NONO Antibody PLA0119 used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.