Rabbit anti-BRCA1 Antibody, Affinity Purified

Name	Rabbit anti-BRCA1 Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0185
Prices	$323.50
Sizes	100 µl
Applications	ICC/IF
Species Reactivities	Human
Gene	BRCA1
Supplier Page	Shop

Product images

<B>Immunohistochemistry</B><BR/>Rabbit Anti-BRCA1 Antibody, Affinity Purified: <B>Cat. No. PLA0185</B>: Detection of Human BRCA1 by Immunohistochemistry. Samples: FFPE section of Human breast carcinoma (left) and ovarian carcinoma (right). Antibody: Affinity purified Rabbit Anti-BRCA1 (<B>Cat. No. PLA0185</B>) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB staining.

Immunohistochemistry
Rabbit Anti-BRCA1 Antibody, Affinity Purified: Cat. No. PLA0185: Detection of Human BRCA1 by Immunohistochemistry. Samples: FFPE section of Human breast carcinoma (left) and ovarian carcinoma (right). Antibody: Affinity purified Rabbit Anti-BRCA1 (Cat. No. PLA0185) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB staining.

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-BRCA1 Antibody, Affinity Purified: <B>Cat. No. PLA0185</B>: Detection of Human BRCA1 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 μg) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-BRCA1 Antibody <B>PLA0185</B> used for WB at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoblotting
Rabbit Anti-BRCA1 Antibody, Affinity Purified: Cat. No. PLA0185: Detection of Human BRCA1 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 μg) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-BRCA1 Antibody PLA0185 used for WB at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

<B>Immunoprecipitation</B><BR/>Rabbit Anti-BRCA1 Antibody, Affinity Purified: <B>Cat. No. PLA0185</B>: Detection of Human BRCA1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-BRCA1 antibody <B>Cat. No. PLA0185</B> used for IP at 6 μg/mg lysate. BRCA1 was also immunoprecipitated by Rabbit Anti-BRCA1 antibody B. For blotting immunoprecipitated BRCA1, <B>PLA0185</B> was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation
Rabbit Anti-BRCA1 Antibody, Affinity Purified: Cat. No. PLA0185: Detection of Human BRCA1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-BRCA1 antibody Cat. No. PLA0185 used for IP at 6 μg/mg lysate. BRCA1 was also immunoprecipitated by Rabbit Anti-BRCA1 antibody B. For blotting immunoprecipitated BRCA1, PLA0185 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.