Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).
ImmunoblottingRabbit Anti-Phospho BRCA1 (S1524) Antibody, Affinity Purified:
Cat. No. PLA0002: Detection of phospho BRCA1 (Ser1524) by Western Blot. Sample: Nuclear extract (50 μg) from HeLa cells mock irradiated or irradiated with 10 Gy of ionizing radiation: Antibody: Affinity purified Rabbit Anti-BRCA1 phospho Ser1524
PLA0002 used at 0.5 μg/mL. Detection: Chemiluminescence.