Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).
ImmunoblottingRabbit Anti-Chk2 Antibody, Affinity Purified:
Cat. No. PLA0122: Detection of Human CHK2 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-CHK2 Antibodies (
Cat. No. PLA0122, and those recognizing different epitopes) were used at 3 μg/mg lysate. For blotting immunoprecipitated CHK2, Antibody was used at 0.04 μg/mL. Detection: Chemiluminescence with an exposure time 30 seconds.