Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).
ImmunoblottingRabbit Anti-DNA-PKcs Antibody, Affinity Purified:
Cat. No. PLA0115: Detection of Human DNA-PKcs by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (15 and 50 μg for WB; 1 mg for IP) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-DNA-PKcs Antibody (
Cat. No. PLA0115) used at 0.1 μg/mL for WB (A and B) and at 3 μg/mg lysate for IP (B). DNA-PKcs was also immunoprecipitated using Rabbit Anti-DNA-PKcs Antibodies that recognize different epitopes. Detection: Chemiluminescence with an exposure time of 30 seconds.