Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).
ImmunoblottingGoat Anti-HP1-beta Antibody, Affinity Purified:
Cat. No. PLA0005: Detection of Human HP1-beta by Western Blot. Samples: Whole cell lysate (NETN extract; 50 μg/lane) from HeLa cells. Primary Antibody: Affinity purified goat Anti-HP1beta Antibody
PLA0005 used at the indicated concentrations. Detection: HRP conjugated Rabbit Anti-goat IgG (1:10,000), a chemiluminescent substrate, and exposure to film for 10 minutes.