Rabbit anti-SRC2 Antibody, Affinity Purified

Name	Rabbit anti-SRC2 Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0094
Prices	$323.50
Sizes	100 µl
Applications	ICC/IF
Species Reactivities	Mouse, Ape, Human, Chimpanzee, Marmoset, Monkey
Gene	NCOA2
Supplier Page	Shop

Product images

<B>Immunohistochemistry</B><BR/>Rabbit Anti-SRC2 Antibody, Affinity Purified: <B>Cat. No. PLA0094</B>: Detection of Human SRC2 by Immunohistochemistry. Sample: FFPE section of Human testicular seminoma. Antibody: Affinity purified Rabbit Anti-SRC2 (<B>Cat. No. PLA0094</B>) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB

Immunohistochemistry
Rabbit Anti-SRC2 Antibody, Affinity Purified: Cat. No. PLA0094: Detection of Human SRC2 by Immunohistochemistry. Sample: FFPE section of Human testicular seminoma. Antibody: Affinity purified Rabbit Anti-SRC2 (Cat. No. PLA0094) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-SRC2 Antibody, Affinity Purified: <B>Cat. No. PLA0094</B>: Detection of Mouse SRC2 by Western Blot. Samples: Whole cell lysate (50 μg) from TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified Rabbit Anti-SRC2 Antibody <B>PLA0094</B> used for WB at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoblotting
Rabbit Anti-SRC2 Antibody, Affinity Purified: Cat. No. PLA0094: Detection of Mouse SRC2 by Western Blot. Samples: Whole cell lysate (50 μg) from TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified Rabbit Anti-SRC2 Antibody PLA0094 used for WB at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

<B>Immunoprecipitation</B><BR/>Rabbit Anti-SRC2 Antibody, Affinity Purified: <B>Cat. No. PLA0094</B>: Immunoprecipitation of Human SRC2. Samples: Nuclear extract (A, 10 mg) or cytosolic (S100) extract (B, 10 mg) from HeLa cells. Antibody: Affinity purified Rabbit Anti-SRC2 (<B>Cat. No. PLA0094</B>) used at 20 μg/10 mg lysate. Detection: Coomassie Brilliant Blue R250 staining of SDS-PAGE gel followed by mass spectrometry to confirm the identity of the band that is SRC2.

Immunoprecipitation
Rabbit Anti-SRC2 Antibody, Affinity Purified: Cat. No. PLA0094: Immunoprecipitation of Human SRC2. Samples: Nuclear extract (A, 10 mg) or cytosolic (S100) extract (B, 10 mg) from HeLa cells. Antibody: Affinity purified Rabbit Anti-SRC2 (Cat. No. PLA0094) used at 20 μg/10 mg lysate. Detection: Coomassie Brilliant Blue R250 staining of SDS-PAGE gel followed by mass spectrometry to confirm the identity of the band that is SRC2.