Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).
ImmunoblottingRabbit Anti-Rad21 Antibody, Affinity Purified:
Cat. No. PLA0022: Detection of Human and Mouse Rad21 by Western Blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg), 293T (Lane T; 50 μg) and Mouse NIH3T3 (Lane M; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-Rad21 Antibody
PLA0022 used for WB at 0.2 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.