Rabbit anti-FLRF/RNF41 Antibody, Affinity Purified

Name	Rabbit anti-FLRF/RNF41 Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0014
Prices	$323.50
Sizes	100 µl
Applications	ICC/IF
Species Reactivities	Bovine, Rabbit, Mouse, Human, Dog, Monkey, Horse, Rat, Guinea Pig, Pig, Primate, Ape
Gene	RNF41
Supplier Page	Shop

Product images

<B>Immunohistochemistry</B><BR/>Rabbit Anti-FLRF/RNF41 Antibody, Affinity Purified: <B>Cat. No. PLA0014</B>: Detection of Human FLRF/RNF41 by Immunohistochemistry. Sample: FFPE section of Human breast duct (upper image) and breast carcinoma (lower image). Antibody: Affinity purified Rabbit Anti-FLRF/RNF41 (<B>Cat. No. PLA0014</B>) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB

Immunohistochemistry
Rabbit Anti-FLRF/RNF41 Antibody, Affinity Purified: Cat. No. PLA0014: Detection of Human FLRF/RNF41 by Immunohistochemistry. Sample: FFPE section of Human breast duct (upper image) and breast carcinoma (lower image). Antibody: Affinity purified Rabbit Anti-FLRF/RNF41 (Cat. No. PLA0014) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-FLRF/RNF41 Antibody, Affinity Purified: <B>Cat. No. PLA0014</B>: Detection of Human and Mouse FLRF/RNF41 by Western Blot and Immunoprecipitation. Samples: Cytoplasmic and membrane proteins (50 μg for WB; 1 mg for IP) from Human monocytic cells (KG1), Mouse multipotent hematopoietic cells (EML), Mouse pre-B cells (70Z3) and Mouse pro-B lymphoid cells (BaF3). Antibody: Affinity purified Rabbit Anti-FLRF/RNF41 Antibody <B>PLA0014</B> used at 0.1 μg/mL for WB (A) and at 5 μg/mg lysate for IP (B). Immunoprecipitated FLRF/RNF41 was detected by WB with affinity purified Rabbit Anti-FLRF/RNF41 Antibody against a different epitope used at 0.1 μg/mL. Detection: Chemilumi-nescence with an exposure time of 20 seconds.

Immunoblotting
Rabbit Anti-FLRF/RNF41 Antibody, Affinity Purified: Cat. No. PLA0014: Detection of Human and Mouse FLRF/RNF41 by Western Blot and Immunoprecipitation. Samples: Cytoplasmic and membrane proteins (50 μg for WB; 1 mg for IP) from Human monocytic cells (KG1), Mouse multipotent hematopoietic cells (EML), Mouse pre-B cells (70Z3) and Mouse pro-B lymphoid cells (BaF3). Antibody: Affinity purified Rabbit Anti-FLRF/RNF41 Antibody PLA0014 used at 0.1 μg/mL for WB (A) and at 5 μg/mg lysate for IP (B). Immunoprecipitated FLRF/RNF41 was detected by WB with affinity purified Rabbit Anti-FLRF/RNF41 Antibody against a different epitope used at 0.1 μg/mL. Detection: Chemilumi-nescence with an exposure time of 20 seconds.