Rabbit anti-KAP-1 Antibody, Affinity Purified

Name	Rabbit anti-KAP-1 Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0078
Prices	$323.50
Sizes	100 µl
Applications	ICC/IF
Species Reactivities	Mouse, Human
Gene	TRIM28
Supplier Page	Shop

Product images

<B>Immunohistochemistry</B><BR/>Rabbit Anti-KAP-1 Antibody, Affinity Purified: <B>Cat. No. PLA0078</B>: Detection of Human KAP-1 by Immunohistochemistry and Immunocytochemistry. Sample: FFPE section of Human prostate carcinoma (left) and formaldelyde-fixed HeLa cells (right). Antibody: Affinity purified Rabbit Anti-KAP-1 (Cat. No.<B>PLA0078</B>) used at a dilution of 1:1,000 (1 μg/mL).Detection: DAB (left) and green fluorescent goat Anti-Rabbit IgG highly cross-adsorbed Antibody FITC conjugated used at a dilution of 1:100 (right).

Immunohistochemistry
Rabbit Anti-KAP-1 Antibody, Affinity Purified: Cat. No. PLA0078: Detection of Human KAP-1 by Immunohistochemistry and Immunocytochemistry. Sample: FFPE section of Human prostate carcinoma (left) and formaldelyde-fixed HeLa cells (right). Antibody: Affinity purified Rabbit Anti-KAP-1 (Cat. No.PLA0078) used at a dilution of 1:1,000 (1 μg/mL).Detection: DAB (left) and green fluorescent goat Anti-Rabbit IgG highly cross-adsorbed Antibody FITC conjugated used at a dilution of 1:100 (right).

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-KAP-1 Antibody, Affinity Purified: <B>Cat. No. PLA0078</B>: Detection of Human KAP1 by Western Blot. Samples: Whole cell lysate from HeLa (5, 15, 50 μg), and 293T (50 μg) cells. Antibodies: Affinity purified Rabbit Anti-KAP1 Antibody <B>PLA0078</B> used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.

Immunoblotting
Rabbit Anti-KAP-1 Antibody, Affinity Purified: Cat. No. PLA0078: Detection of Human KAP1 by Western Blot. Samples: Whole cell lysate from HeLa (5, 15, 50 μg), and 293T (50 μg) cells. Antibodies: Affinity purified Rabbit Anti-KAP1 Antibody PLA0078 used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.

<B>Immunoblotting</B><BR/>Rabbit Anti-KAP-1 Antibody, Affinity Purified: <B>Cat. No. PLA0078</B>: Detection of Mouse KAP1 by Western Blot. Samples: Whole cell lysate from TCMK-1 (50 μg) cells. Antibodies: Affinity purified Rabbit Anti-KAP1 Antibody <B>PLA0078</B> used for WB at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoblotting
Rabbit Anti-KAP-1 Antibody, Affinity Purified: Cat. No. PLA0078: Detection of Mouse KAP1 by Western Blot. Samples: Whole cell lysate from TCMK-1 (50 μg) cells. Antibodies: Affinity purified Rabbit Anti-KAP1 Antibody PLA0078 used for WB at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.