Goat anti-KAP-1 Antibody, Affinity Purified

Name	Goat anti-KAP-1 Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0295
Prices	$323.50
Sizes	100 µl
Applications	IP
Species Reactivities	Human
Gene	TRIM28
Supplier Page	Shop

Product images

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Goat Anti-KAP-1 Antibody, Affinity Purified: <B>Cat. No. PLA0295</B>: Detection of Human KAP-1 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 μg), HeLa (Lane H; 50 μg) and Jurkat (J; 50 μg) cells. Antibodies: Affinity purified goat Anti-KAP-1 Antibody <B>PLA0295</B> used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoblotting
Goat Anti-KAP-1 Antibody, Affinity Purified: Cat. No. PLA0295: Detection of Human KAP-1 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 μg), HeLa (Lane H; 50 μg) and Jurkat (J; 50 μg) cells. Antibodies: Affinity purified goat Anti-KAP-1 Antibody PLA0295 used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

<B>Immunoprecipitation</B><BR/>Goat Anti-KAP-1 Antibody, Affinity Purified: <B>Cat. No. PLA0295</B>: Detection of Human KAP-1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified goat Anti-KAP-1 antibody <B>Cat. No. PLA0295</B> used for IP at 6 μg/mg lysate. KAP-1 was also immunoprecipitated by Rabbit Anti-KAP-1 Antibody <B>Cat. No. PLA0078</B>. For blotting immunoprecipitated KAP-1, <B>PLA0295</B> was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.

Immunoprecipitation
Goat Anti-KAP-1 Antibody, Affinity Purified: Cat. No. PLA0295: Detection of Human KAP-1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified goat Anti-KAP-1 antibody Cat. No. PLA0295 used for IP at 6 μg/mg lysate. KAP-1 was also immunoprecipitated by Rabbit Anti-KAP-1 Antibody Cat. No. PLA0078. For blotting immunoprecipitated KAP-1, PLA0295 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.