Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).
ImmunoblottingRabbit Anti-SMC4 Antibody, Affinity Purified:
Cat. No. PLA0020: Detection of Human SMC4 by Western Blot and Immuno-precipitation. Samples: Whole cell lysate (90 μg for WB; 0.5 mg for IP/WB) from 293T cells (lanes 1, 3 and 5), normal Human lymphoblasts (lane 2) or SMC4 transfected 239T cells (lanes 4, 6 and 7) separated on an 8% SDS-PAGE gel. Antibodies: Affinity purified Rabbit Anti-SMC4
PLA0020 used at 0.2 or 0.1 μg/mL for WB and 5 μg for IP. Detection: Chemiluminescence with the indicated exposure times.