Rabbit anti-BMI1 Antibody, Affinity Purified

Name	Rabbit anti-BMI1 Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0208
Prices	$323.50
Sizes	100 µl
Applications	ICC/IF
Species Reactivities	Rabbit, Guinea Pig, Mouse, Chicken, Rat, Amphibians, Bovine, Horse, Human, Primate, Dog, Pig
Gene	BMI1
Supplier Page	Shop

Product images

<B>Immunohistochemistry</B><BR/>Rabbit Anti-BMI1 Antibody, Affinity Purified: <B>Cat. No. PLA0208</B>: Detection of Human BMI1 by Immunohistochemistry. Sample: FFPE section of Human lung carcinoma. Antibody: Affinity purified Rabbit Anti-BMI1 <B>Cat. No. PLA0208</B> used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB.

Immunohistochemistry
Rabbit Anti-BMI1 Antibody, Affinity Purified: Cat. No. PLA0208: Detection of Human BMI1 by Immunohistochemistry. Sample: FFPE section of Human lung carcinoma. Antibody: Affinity purified Rabbit Anti-BMI1 Cat. No. PLA0208 used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB.

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-BMI1 Antibody, Affinity Purified: <B>Cat. No. PLA0208</B>: Detection of Human and Mouse BMI1 by Western Blot. Samples: Whole cell lysate (50 μg) from HeLa, 293T, Jurkat, Mouse TCMK-1, and Mouse NIH3T3 cells. Antibodies: Affinity purified Rabbit Anti-BMI1 Antibody <B>PLA0208</B> used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.

Immunoblotting
Rabbit Anti-BMI1 Antibody, Affinity Purified: Cat. No. PLA0208: Detection of Human and Mouse BMI1 by Western Blot. Samples: Whole cell lysate (50 μg) from HeLa, 293T, Jurkat, Mouse TCMK-1, and Mouse NIH3T3 cells. Antibodies: Affinity purified Rabbit Anti-BMI1 Antibody PLA0208 used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.

<B>Immunoprecipitation</B><BR/>Rabbit Anti-BMI1 Antibody, Affinity Purified: <B>Cat. No. PLA0208</B>: Detection of Human BMI1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-BMI1 antibody <B>Cat. No. PLA0208</B> used for IP at 6 μg/mg lysate. BMI1 was also immunoprecipitated by BMI1 antibody B. Detection: Chemiluminescence with an exposure time of 10 seconds.

Immunoprecipitation
Rabbit Anti-BMI1 Antibody, Affinity Purified: Cat. No. PLA0208: Detection of Human BMI1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-BMI1 antibody Cat. No. PLA0208 used for IP at 6 μg/mg lysate. BMI1 was also immunoprecipitated by BMI1 antibody B. Detection: Chemiluminescence with an exposure time of 10 seconds.