Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).
ImmunoblottingGoat Anti-BRD2 Antibody, Affinity Purified:
Cat. No. PLA0066: Detection of Human BRD2 by Western Blot. Sample: RIPA extract (10 μg) from HeLa cells that was untreated (-) or treated (+) with CIP (calf intestinal phosphatase). Antibody: Affinity purified goat Anti-BRD2 (
Cat. No. PLA0066) used at 0.05 μg/mL followed by Rabbit Anti-goat HRP (A50-100P, 1:20,000). Detection: Chemiluminescence with 1 minute exposure.