Rabbit anti-BRD2 Antibody, Affinity Purified

Name	Rabbit anti-BRD2 Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0261
Prices	$323.50
Sizes	100 µl
Applications	ICC/IF
Species Reactivities	Dog, Horse, Chimpanzee, Marmoset, Mouse, Bovine, Monkey, Ape, Pig, Human, Primate
Gene	BRD2
Supplier Page	Shop

Product images

<B>Immunohistochemistry</B><BR/>Rabbit Anti-BRD2 Antibody, Affinity Purified: <B>Cat. No. PLA0261</B>: Detection of Human BRD2 by Immunohistochemistry. Sample: FFPE section of Human breast carcinoma. Antibody: Affinity purified Rabbit Anti-BRD2 (Cat. No.<B>PLA0261</B>) used at a dilution of 1:200 (1 μg/mL). Detection: DAB staining.

Immunohistochemistry
Rabbit Anti-BRD2 Antibody, Affinity Purified: Cat. No. PLA0261: Detection of Human BRD2 by Immunohistochemistry. Sample: FFPE section of Human breast carcinoma. Antibody: Affinity purified Rabbit Anti-BRD2 (Cat. No.PLA0261) used at a dilution of 1:200 (1 μg/mL). Detection: DAB staining.

<B>Immunocytochemistry</B><BR/>Rabbit Anti-BRD2 Antibody, Affinity Purified: <B>Cat. No. PLA0261</B>: Detection of Human BRD2 by Immunocytochemistry. Sample: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified Rabbit Anti-BRD2 (Cat. No.<B>PLA0261</B>) used at a dilution of 1:100 (2 μg/mL). Detection: Red-fluorescent goat Anti-Rabbit IgG cross-adsorbed Antibody DyLight<SUP>®</SUP> 594 conjugated.

Immunocytochemistry
Rabbit Anti-BRD2 Antibody, Affinity Purified: Cat. No. PLA0261: Detection of Human BRD2 by Immunocytochemistry. Sample: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified Rabbit Anti-BRD2 (Cat. No.PLA0261) used at a dilution of 1:100 (2 μg/mL). Detection: Red-fluorescent goat Anti-Rabbit IgG cross-adsorbed Antibody DyLight^® 594 conjugated.

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-BRD2 Antibody, Affinity Purified: <B>Cat. No. PLA0261</B>: Detection of Human BRD2 by Western Blot. Samples: Whole cell lysate (50 μg) from HeLa and 293T cells. Antibodies: Affinity purified Rabbit Anti-BRD2 Antibody (<B>Cat. No. PLA0261</B>) used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoblotting
Rabbit Anti-BRD2 Antibody, Affinity Purified: Cat. No. PLA0261: Detection of Human BRD2 by Western Blot. Samples: Whole cell lysate (50 μg) from HeLa and 293T cells. Antibodies: Affinity purified Rabbit Anti-BRD2 Antibody (Cat. No. PLA0261) used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

<B>Immunoprecipitation</B><BR/>Rabbit Anti-BRD2 Antibody, Affinity Purified: <B>Cat. No. PLA0261</B>: Detection of Human BRD2 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit Anti-BRD2 antibody <B>Cat. No. PLA0261</B> used for IP at 6 μg per reaction. BRD2 was also immunoprecipitated by BRD2 Antibody B. For blotting immunoprecipitated BRD2, <B>PLA0261</B> was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation
Rabbit Anti-BRD2 Antibody, Affinity Purified: Cat. No. PLA0261: Detection of Human BRD2 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit Anti-BRD2 antibody Cat. No. PLA0261 used for IP at 6 μg per reaction. BRD2 was also immunoprecipitated by BRD2 Antibody B. For blotting immunoprecipitated BRD2, PLA0261 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.