ImmunohistochemistryRabbit Anti-Phospho MCM2 (S41) Antibody, Affinity Purified:
Cat. No. PLA0034: Detection of Human and Mouse MCM2 (Ser41) by Immunohistochemistry. Sample: FFPE section of Human laryengeal squamous cell carcinoma (left) and Mouse squamous cell carcinoma (right). Antibody: Affinity purified Rabbit Anti-MCM2 (Ser41) (
Cat. No. PLA0034) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB
Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).
ImmunoblottingRabbit Anti-Phospho MCM2 (S41) Antibody, Affinity Purified:
Cat. No. PLA0034: Detection of Human Phospho MCM2 (Ser41) by Western Blot. Samples: Whole cell lysate (30 μg) from HeLa cells that were either untreated or treated with alkaline phosphatase (AP). Antibodies: Affinity purified Rabbit Anti-phospho MCM2 (Ser41) Antibody
PLA0034 used at 0.1 μg/mL. After probing with the phospho-specific Antibodies, Rabbit Anti-MCM2 Antibody
PLA0060 was used at 0.2 μg/mL to show total MCM2. Detection: Chemiluminescence with a 1 minute exposure.