Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).
ImmunoblottingRabbit Anti-CBP Antibody, Affinity Purified:
Cat. No. PLA0097: Detection of Human CBP by Western Blot. Samples: Whole cell lysate (5, 15 and 50 μg) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-CBP Antibody
PLA0097 used for WB at 0.4 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
ImmunoprecipitationRabbit Anti-CBP Antibody, Affinity Purified:
Cat. No. PLA0097: Detection of Human CBP by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-CBP antibody
Cat. No. PLA0097 used for IP at 6 μg/mg lysate. CBP was also immunoprecipitated by Rabbit Anti-CBP antibody B. For blotting immunoprecipitated CBP,
PLA0097 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.