Rabbit anti-USP7 Antibody, Affinity Purified

Name	Rabbit anti-USP7 Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0009
Prices	$323.50
Sizes	100 µl
Applications	ICC/IF
Species Reactivities	Pig, Mouse, Rat, Ape, Human, Monkey, Zebra finch
Gene	USP7
Supplier Page	Shop

Product images

<B>Immunohistochemistry</B><BR/>Rabbit Anti-USP7 Antibody, Affinity Purified: <B>Cat. No. PLA0009</B>: Detection of Human by Immunohistochemistry. Sample: FFPE section of Human stomach carcinoma with citrate buffer pH 6.0 epitope retrieval. Antibody: Affinity purified Rabbit Anti-USP7 (<B>Cat. No. PLA0009</B>) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB

Immunohistochemistry
Rabbit Anti-USP7 Antibody, Affinity Purified: Cat. No. PLA0009: Detection of Human by Immunohistochemistry. Sample: FFPE section of Human stomach carcinoma with citrate buffer pH 6.0 epitope retrieval. Antibody: Affinity purified Rabbit Anti-USP7 (Cat. No. PLA0009) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-USP7 Antibody, Affinity Purified: <B>Cat. No. PLA0009</B>: Detection of Human and Mouse USP7 by Western Blot. Samples: Whole cell lysate (50 μg) from HeLa, 293T, Jurkat, Mouse TCMK-1, Mouse NIH3T3 cells. Antibodies: Affinity purified Rabbit Anti-USP7 Antibody <B>PLA0009</B> used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoblotting
Rabbit Anti-USP7 Antibody, Affinity Purified: Cat. No. PLA0009: Detection of Human and Mouse USP7 by Western Blot. Samples: Whole cell lysate (50 μg) from HeLa, 293T, Jurkat, Mouse TCMK-1, Mouse NIH3T3 cells. Antibodies: Affinity purified Rabbit Anti-USP7 Antibody PLA0009 used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

<B>Immunoprecipitation</B><BR/>Rabbit Anti-USP7 Antibody, Affinity Purified: <B>Cat. No. PLA0009</B>: Detection of Human USP7 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit Anti-USP7 antibody <B>Cat. No. PLA0009</B> used for IP at 6 μg per reaction. USP7 was also immunoprecipitated by Rabbit Anti-USP7 antibody B. For blotting immunoprecipitated USP7, <B>Cat. No. PLA0009</B> was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation
Rabbit Anti-USP7 Antibody, Affinity Purified: Cat. No. PLA0009: Detection of Human USP7 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit Anti-USP7 antibody Cat. No. PLA0009 used for IP at 6 μg per reaction. USP7 was also immunoprecipitated by Rabbit Anti-USP7 antibody B. For blotting immunoprecipitated USP7, Cat. No. PLA0009 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.