Goat anti-USP7 Antibody, Affinity Purified

Name	Goat anti-USP7 Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0306
Prices	$323.50
Sizes	100 µl
Applications	IP
Species Reactivities	Human, Mouse
Gene	USP7
Supplier Page	Shop

Product images

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Goat Anti-USP7 Antibody, Affinity Purified: <B>Cat. No. PLA0306</B>: Detection of Human USP7 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 μg), HeLa (50 μg), and Jurkat (50 μg) cells. Antibodies: Affinity purified goat Anti-USP7 Antibody <B>PLA0306</B> used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoblotting
Goat Anti-USP7 Antibody, Affinity Purified: Cat. No. PLA0306: Detection of Human USP7 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 μg), HeLa (50 μg), and Jurkat (50 μg) cells. Antibodies: Affinity purified goat Anti-USP7 Antibody PLA0306 used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

<B>Immunoblotting</B><BR/>Goat Anti-USP7 Antibody, Affinity Purified: <B>Cat. No. PLA0306</B>: Detection of Mouse USP7 by Western Blot. Samples: Whole cell lysate (50 μg) from NIH3T3, Renca, and TCMK-1 cells. Antibodies: Affinity purified goat Anti-USP7 Antibody <B>PLA0306</B> used for WB at 0.4 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoblotting
Goat Anti-USP7 Antibody, Affinity Purified: Cat. No. PLA0306: Detection of Mouse USP7 by Western Blot. Samples: Whole cell lysate (50 μg) from NIH3T3, Renca, and TCMK-1 cells. Antibodies: Affinity purified goat Anti-USP7 Antibody PLA0306 used for WB at 0.4 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

<B>Immunoprecipitation</B><BR/>Goat Anti-USP7 Antibody, Affinity Purified: <B>Cat. No. PLA0306</B>: Detection of Human USP7 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified goat Anti-USP7 antibody <B>Cat. No. PLA0306</B> used for IP at 6 μg/mg lysate. USP7 was also immunoprecipitated by Rabbit Anti-USP7 antibody <B>Cat. No.PLA0009</B>. For blotting immunoprecipitated USP7, <B>Cat. No. PLA0009</B> was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation
Goat Anti-USP7 Antibody, Affinity Purified: Cat. No. PLA0306: Detection of Human USP7 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified goat Anti-USP7 antibody Cat. No. PLA0306 used for IP at 6 μg/mg lysate. USP7 was also immunoprecipitated by Rabbit Anti-USP7 antibody Cat. No.PLA0009. For blotting immunoprecipitated USP7, Cat. No. PLA0009 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.